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Effect of temperature and pH on the activity of EstCS2. (a) Enzyme activity was measured at each temperature under standard assay condition (■). In addition, the enzyme was preincubated at the indicated temperature, and the remaining activity was determined (●). (b) Enzyme activity was measured at each pH under standard assay conditions (■). In addition, the enzyme was preincubated at the indicated pH, and the remaining activity was determined (●).
