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. 2011 May 24;9:59. doi: 10.1186/1477-7819-9-59

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Copyright ©2011 Chen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sequence-specific and dose-dependent inhibition of clusterin expression by AS clusterin ODN in BxPc-3 cells. In A, BxPc-3 cells were treated daily with various concentrations of AS cluserin ODN or a 2-base clusterin MM ODN as a control for 2 days, total cell protein was extracted from culture cells, and clusterin and GAPDH levels were analyzed by western blotting. In B, quantitative analysis of clusterin protein levels after normalization to GAPDH levels in BxPc-3 cells after treatment with various concentrations of AS clusterin ODN or MM control ODN was performed using laser densitometry. Each point represents the mean of triplicate analyses with SD. *, differs from controls (P < 0.01) by Student's t test.