Table 2.
Sequencing of wild type and mutated alleles with a particular program of pyrosquencing
| nucleotide dispensation during pyrosequencing | G | T | A | T | C | A | G | A | C | A | T | G | A | C | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | T | A | T | C | AA | GG | AA | TT | AA | ||||||
| allelic | c.2235-2249del | T | A | T | C | AAAA | C | A | T | C | |||||
| sequence of | c.2236-2250del | T | A | T | C | AA | G | A | C | A | T | C | |||
| c.2237-2251del | T | A | T | C | AA | GG | C | A | T | C | |||||
| c.2240-2257del | T | A | T | C | AA | GG | AA | T | C | ||||||
Bold letters correspond to the nucleotides identical in wild type and mutated alleles; italic letters correspond to the nucleotides specific of mutated alleles.