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. 2011 Jun 13;18(1):38. doi: 10.1186/1423-0127-18-38

Figure 1.

Figure 1

Effect of Ang II and losartan on PLCβ1 Tyr-phosphorylation and SHP-2 content. Fibroblasts, cultured in absence or presence of Ang II (100 nM) and Ang II (100 nM) plus losartan (100 μM), were scraped and extracted with buffer C (see methods). Total cell lysate (200 μg) were immunoprecipitated with anti-PLCβ1 antibody. Immunoprecipitates were subjected to Western blotting (analysed by 10% SDS/PAGE gels) and immunorevealed with mouse-anti-PLCβ1 P-Tyr (A) and rabbit-anti-SHP-2 (B) antibodies, before being stripped and immunorevealed with anti-PLCβ1 (mouse) (C). The figure is representative of five separate experiments carried out in duplicate. Panels D and E present the percent change relative to unstimulated cells ± SD (N = 5 experiments) for panel A and B respectively. Panel D: **: p < 0.0001 vs Basal; *: p = 0.003 vs Ang. Panel E: ***: p < 0.0001 vs Basal; *: p = 0.006 vs Ang+Los; **: p = 0.002 vs Basal.