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. 2011 Jun 13;18(1):38. doi: 10.1186/1423-0127-18-38

Figure 2.

Figure 2

Effect of Ang II on SHP-2 association with PLCβ1 and SHP-2 Tyr-phosphorylation. Total cell lysate (200 μg) were immunoprecipitated with anti-SHP-2 antibody. Immunoprecipitates were subjected to Western blotting (analysed by 8% SDS/PAGE gels) and immunorevealed with mouse-anti-PLCβ1 (A), rabbit-anti-phospho tyrosine SHP-2 (B) antibodies, rabbit anti SHP2(C). The figure is representative of five separate experiments carried out in duplicate. Panels D and E present the percent change relative to unstimulated cells and SD (N = 5 experiments) for panel A and B respectively. Panel D: **: p < 0.0001 vs Basal; *: p = 0.001 vs Ang+Los Panel E: *: p < 0.0001 vs Basal; +: p < 0.0001 vs Ang+Los.