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. 2011 May 13;8:36. doi: 10.1186/1742-4690-8-36

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Copyright ©2011 Hayes et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Host miRNA expression levels were compared between HIV-1^NL4-3, Vif/Vpr-deficient or Tat K51A RSS-deficient strains. CEMx174 lymphocytes were infected by co-culture with HIV-1^NL4-3, HIV-1^NL4-3ΔVV that contains a premature stop codon in vif and frameshift in vpr, or HIV-1^NL4-3RSS that contains the K51A substitution that eliminates Tat RSS activity. Total cellular RNA was reverse transcribed and hybridized to miRNA microarray chips with two or three independent biological replicates to determine relative expression levels of 518 mature miRNA and 336 precursor miRNA that were monitored by 906 human miRNA probes spotted in duplicate [53].