Figure 3.
Colocalization of TRIM5α and TRIM19 (PML) in leptomycin B-treated cells. HeLa cells stably expressing HA-tagged TRIM5αrh proteins were treated with 5 ng/ml of leptomycin B (LMB) for two hours. Cells were stained for TRIM5α using anti-HA FITC-conjugated antibodies. PML was stained using anti-PML antibodies and Cy3-conjugated anti-goat secondary antibodies (A). LMB-treated HeLa cells expressing TRIM5αrh were fixed. Ultrathin sections were labeled using an anti-HA antibody and Protein A-gold (10-nm particles). Ring-like structures (n, nuclear bodies) in the cell nucleus were labeled with the antibody (B).