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Leptin induces circulating MSH in ob/ob mutants. Ob/ob mutant mice (ob/ob) and age-matched controls (wild type and heterozygotes, +/?), 10 per group, were injected i.p. with 0.1 ml of either 7 μg leptin (lep) or vehicle (PBS) alone (veh). One hour after the leptin injection blood was collected retroorbitally into tubes containing EDTA. Plasma was assayed for MSH by RIA.
