Table 1. Details of the six fragments used in the S. aureus typing scheme.

Fragment name	SNP(s) position(s) in concatentated MLST sequence	Region interrogated by HRM	Primers (5′-3′)	Fragment size (bp)	HRM normalisation regions (°C)	Number of predicted curves	Predicted Δ Tm (°C)
arcC78/210	78, 210	73–210	TGGATACTTGTGGTGCAATG	181	74–75	6	0.23
			CGTATAAAAAGGACCAATTGGTTT		80–81
aroE88/155	543, 610	521–617	TAAATATTCCAATTGAAGATTTTC	140	67–68	6	0.29
			CTGCATTAATCGCTTGTTCA		76–77
gmk286	1663	1637–1680	GAAGTAGAAGGTGCAAAGC	83	69–70	4	0.49
			CAAGTGATCTAAACTTGGAGG		77–78
pta294	2100	2100–2216	TGCAGCACATTCAACAGG	158	74–75	5	0.26
			CCTTGTGAATCAAGTTCTGGATTG		82–83
tpi36	2316	2316–2485	GATGAAGAAATTAACAAAAAAGCGCA	219	74–75	6	0.19
			TTGATGATTTACCAGTTCCGATTG		81–82
tpi241/243	2521, 2523	2521–2644	GTAAATCATCAACATCTGAAGAT	168	73–74	4	0.24
			GCCCCATCAATATCAGTTTGTG		79–80

The normalization regions refer to the temperatures selected to normalize the florescence curves using the Corbett Rotorgene software.