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. 2011 Jun 22;6(6):e21019. doi: 10.1371/journal.pone.0021019

Figure 2. Coffea gametophytic self-incompatibility.

Figure 2

The strength of the Coffea GSI response was measured by examining pollen tube growth using analine blue staining. A. Self-pollen tube growth in SI C. perrieri in cultivation at the Kianjavato Coffee Research Station, Madagascar. Pollen tubes terminate shortly after entering the style in bulbous callose plugs that fluoresce brightly. B. Self-pollen tube growth in SC C. arabica in cultivation at the Duke University Department of Biology Greenhouse Facility. Pollen tubes can be seen growing through the style tissue unhindered, leading to self-fertilization.