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. 2011 Jun 22;6(6):e21019. doi: 10.1371/journal.pone.0021019

Figure 6. Expression of RNase T2 genes in C. arabica tissues.

Figure 6

Agarose gel image showing RT-PCR results using gene-specific primers for Coffea RNase “A”, “C”, and S-RNase genes. cDNAs were generated from RNA extracts of various C. arabica tissues labeled as follows: P1 = pre-anthesis pistils dissected from flower buds; P2 = post-anthesis pistils; L1 = leaf at 1 week; L2 = leaf at >2 weeks; R1 = fine root; R2 = primary root; F1 = fruit at approximately 1 week; F2 = fruit at >3 weeks. RNase “A” and “C” genes are constitutively expressed in C. arabica tissues, but the S-RNase gene shows pistil-specific expression characteristic of genes involved in the S-RNase GSI system.