Figure 3. Discovery of a region adjacent to the ING1b-plant homeodomain (PHD) that acts as a ubiquitin-binding domain (UBD).

A ) Lysates from Hs68 cells treated or transfected as indicated and blotted for p53. Lower panels confirm ING1-expression and loading. B ) Cells treated as in A), but also co-infected with p53-adenovirus, were blotted as in A). Abbreviations: CBB: Coomassie-stained loading-controls; GFP: green fluorescent protein, ING1b ^del(PHD-PBR): ING1b-construct with PHD-PBR deletion, PBR: polybasic region, wt-wild-type.