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. 2011 Jun 22;6(6):e21214. doi: 10.1371/journal.pone.0021214

Figure 2. Naïve B16 specific Pmel-1 T cells are primed by tumor growth and infiltrate tumors after adoptive transfer into tumor bearing mice.

Figure 2

2.5×105 B16 tumor cells were inoculated in matrigel into WT C57B/6 mice. Naive CFSE labeled Thy 1.1+ CD8+ Pmel-1 T cells were AT either 6 days (A, B) or 3 days (C) after tumor challenge. Spleens, TDLNs and tumors were isolated from 3 mice every 24–48 hours after AT for up to 11 days post transfer and analyzed for Pmel-1 T cell infiltration and activation (A) LEFT, representative SSC/Thy 1.1 FACS plots from 7 days post AT of 2×106 naïve Pmel-1 T cells show percentages of infiltrating Thy 1.1+ Pmel-1 T cells. RIGHT panels shows CD44/CFSE, CD62L/CFSE on noted days post AT. Gates show representative percentages of activated (CD44High/CFSELow, or CD62LLow/CFSELow) Pmel-1 T cells infiltrating tissues at each time point. (B, C) Mean +/− SEM absolute numbers (left) and CFSE phenotype percentages (right) calculated from the SSClow Thy1.1+. gate. (B) AT of 2×106 CD8+ Pmel-1 T cells (C) AT of 3×105 CD8+ Pmel-1 T cells Percentages shown are representative of 2–3 independent experiments with 3 mice/timepoint.