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. 2011 Jun 22;6(6):e21187. doi: 10.1371/journal.pone.0021187

Figure 1. Effect of dietary leucine on body weight, fat mass and food consumption.

Figure 1

Eight week-old male C67BL/6 mice were fed a chow diet or a high fat diet, with or without supplementation by leucine (1.5% w/v) in the drinking water for 8 weeks as described in Methods. A) Leucine intake was calculated based on the amount of leucine consumed in the drinking water and the amount contained in the food as provided by the manufacturers (25 mice per group). B) Histogram plots showing relative levels of serum leucine as measured by UHPLC-MS/MS at the end of the eighth week with 8–10 mice per group. C) Body weights were monitored at the weeks indicated (25 mice per group). D) Tissue weight was measured at the end of the 8 weeks on each diet (5 mice per group). E) Food intake was measured every week and the mean consumption was calculated for every dietary intervention (25 mice per group).