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. 2011 Jun 22;6(6):e21187. doi: 10.1371/journal.pone.0021187

Figure 2. Leucine supplementation improves glucose tolerance and hepatic steatosis in mice on HFD.

Figure 2

A) Glucose tolerance tests were performed after an overnight fast in the three study groups. Mice received an intraperitoneal injection of 2 g/kg body wt glucose. Glucose was measured in tail vein blood samples at the indicated times. The area under the curve (AUC) was calculated for each dietary condition. Data points are means ± SEM with 7–8 mice in each group. B) Mice were sacrificed and livers were harvested after 8 weeks on each diet. The livers were then formalin fixed, embedded in paraffin and sections stained with H&E. Three mouse livers per group were analyzed. C) mRNA was extracted from 200 mg liver and subjected to quantitative real time PCR. Gene expression for FAS and ACC were normalized against TATA-binding protein (TBP). 5 livers per group were used. *P<0.05.