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. 2011 Jun 22;6(6):e21299. doi: 10.1371/journal.pone.0021299

Figure 3. Sucrose fermentation of SX-4 and SX-4-pBADVCA0654-6.

Figure 3

(A) Sucrose fermentation in V. mimicus; the red portion shows the function of the enzyme translated from VCA0654 to VCA0656 in the pathway. (B) Complement test. The gene cluster of VCA0654 to VCA0656 from V. cholerae strain N16961 was complemented into the V. mimicus strain SX-4. The strain was grown under LB in the presence of 0.1% arabinose, and then tested for sucrose fermentation (API-20E). Only the strain bearing plasmid pBADVCA0654-6 presented a positive result, like that of V. cholerae N16961. (C) Genome structure of the VCA0654-VCA0655-VCA0656 operon. Positions and orientations of ORFs are indicated with different color arrows. Homologue genes are presented with the same color. Homologue regions have been showed with red background, also with their flanking sequences. Insertions in VM603 and MB-451 are homologues presenting with the same color.