Figure 3. Radioactive assay for activity measurements of Diels-Alderase ribozymes.

The picture shows thin-layer plates for the thermal background reaction (left) and the RNA catalyzed reaction (right) scanned on a Typhoon imager for radioactivity. For each point in time in minutes, one aliquot of the reaction mixture (0.7 µl) was spotted. Abbreviations are R = reference (AHEG-³²P alone), DAP-³²P = Diels-Alder product spots, AHEG-³²P = starting material spots and α-³²P-AMP = internal standard spots.