Figure 5. Reaction kinetics of dye-labeled Diels-Alderase ribozyme constructs determined by the radiometric assay.

(A) Reaction kinetics of constructs with the first selection of dye-positions. Conditions: 7.0 µM RNA, 1.0 mM AHEG-³²P and 1.0 mM NPM. (B) Reaction kinetics of constructs with optimized dye-positions, employing 0.1 mM AHEG-³²P. References (black) are the reaction of the wild-type ribozyme (wt DAse) and the background reaction without RNA.