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. 2011 Jun 22;6(6):e21400. doi: 10.1371/journal.pone.0021400

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(A) An incorrectly inverted contig (red) moves two exons (exons 14 and 15) to the wrong strand, causing the annotation software to miss them. Instead, it used low-quality alignments on the wrong strand, creating frameshifts in the predicted protein sequence that contained multiple premature stop codons. (B) Sequence alignment of the predicted AQR protein (conceptual translation) and its RefSeq model.