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. 2011 Jun 22;6(6):e21372. doi: 10.1371/journal.pone.0021372

Figure 5. PorT-dependent glycosylation of GFP-CTD fusion proteins.

Figure 5

(A) Diagram of a GFP-CTD fusion protein expressed in P. gingivalis. The fusion protein consists of a signal sequence from HBP35, a GFP domain and a CTD. An 85 amino acid C-terminal region containing the CTD was used for HBP35, CPG70, peptidylarginine deiminase (PAD) and P27, and one with 80 amino acids was used for RgpB. (B) Anti-GFP immunoblot analysis of P. gingivalis strains 33277 (WT) and KDP117 (porT) expressing GFP-CTD fusion proteins.