Fig.3.

Time course expression and secretion of CT311 protein during C. trachomatis infection. The C. tracomatis-infected HeLa cell samples were processed at various times after infection (as indicated on the top) for immunofluorescence staining as described in the legend to Fig. 1. The anti-CT311 (mAb 7C10; panels a to i), anti-CPAF (mAb 100a; j to r) were visualized with a goat anti-mouse IgG conjugated with Cy3 (red) while the chlamydial organisms were visualized with a rabbit anti-chlamydia antibody plus a goat anti-rabbit IgG-Cy2 conjugate (green). Images amplified in separate panels were marked with white squares and the corresponding amplified images were labeled the same letters followed by the number 1. Note that both CT311 (d & d1) and CPAF (m & m1) proteins were first detected associated with the chlamydial inclusions at 12h (red arrowheads) while secretion out of the inclusions was first detected at 24h (for CT311, panels f & f1) and 18h (for CPAF, n & n1) post infection respectively (red arrows). The secreted CT311 and CPAF remained in the infected cells throughout the infection cycle. The experiments were repeated 3 times.