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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Microb Pathog. 2011 May 7;51(3):121–132. doi: 10.1016/j.micpath.2011.04.010

Fig 3.

Fig 3

Bacterial growth of all the mutants in the presence of rBALF or LL-37. (A) Bacteria were grown in BHI to an A600 of 0.3 at 37°C, diluted to an A 600 of 0.08, rBALF was added to a concentration of 60 μg/ml protein and the cultures were further incubated for 18 h before measuring growth (A600). MICs were determined in microtiter plates and were 60μg/ml protein for DSY101, all the polymyxin B mutants, PM2-3 and PM3-2, and 120 μg/ml protein for PM2-49, PM5-6 and PM12-3. (B) Bacteria were grown in BHI to an A600 of 0.3 at 37°C, diluted to an A 600 of 0.08, LL-37 was added to 20, 40, 80 or 160 μg/ml protein and the cultures were further incubated for 18 h before determining bacterial survival (CFUs). All the data are means of at least three independent experiments with standard errors.