Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source |
|---|---|---|
| E. coli | ||
| CC118λpir | Δ(ara-leu) ΔaraD lacX74 galE galK phoA thi-1 rpsE rpoB argE(Am) recA1, lysogenized with λpir phage | [74] |
| DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Invitrogen Corp., Carlsbad, CA |
| BL21(DE3) | E. coli B (DE3)[F− dcm ompT hsdSB (rB− mB−)] | Novagen, EMD Biosc., Madison, WI |
| Y. pestis | ||
| KIM5 | Y. pestis KIM pgm | [75] |
| KIM6 | KIM5 pCD1− (LCR−) | [75] |
| DSY88 | KIM6 Δcaf Δpsa pPCP1ΩaphA Δpla | [48] |
| DSY101 | DSY88 PCP1Δpla | This study |
| DSY131 | DSY101 Δy1522::aphA | This study |
| DSY132 | DSY101 Δy1523::aphA | This study |
| DSY133 | DSY131 pTrc99A | This study |
| DSY134 | DSY131 pHcpy1522 | This study |
| DSY135 | DSY101 Δy3910(feoC)::aphA | This study |
| DSY136 | DSY101 Δy3910–y3911(feoBC)::aphA | This study |
| DSY137 | KIM5 Δy3911(feoB)::aphA | This study |
| Plasmids | ||
| pNJ17 | TnAraOut delivery vehicle; Kmr | [65] |
| pRL27 | Plasmid with mini-Tn5 and oriR6K; Kmr | [66] |
| pTn5AraOut | pRL27 with araC-PBAD from pNJ17; Kmr | This study |
| pRS415 | lacZ transcriptional fusion vector; Apr | [76] |
| pSIM9 | pRK2 gam exo bet; Cmr | [77] |
| pKD4 | Plasmid with the Kmr template for PCR | [72] |
| pET22b(+) | Expression vector; His6 affinity tag; Apr | Novagen |
| pETHcp | pET22b(+)-y1522; Apr | This study |
| pTrc99A | Expression vector with trc (trp-lac) promoter; Apr | [78] |
| pHcpy1522 | pTrc99A-y1522 | This study |
| pFeoAB | pTrc99A-y3911–y3912 | This study |