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Transfection of pUbAcAtg3g for the intracellular localization of Atg3. Expressed Atg3-EGFP showed dispersed fluorescence in the cytoplasm in the trophozoites (encystation-0 hr) and the early stage of encystation (encystation-20 hr). After incubation for 40 hr in encystment media, AcAtg3-EGFP localized around autophagosomal membrane (encystation-40 hr).
