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. 2001 Mar 13;98(7):4249–4254. doi: 10.1073/pnas.061437498

Figure 1.

Figure 1

Three barley 14-3-3 isoforms are present within the mitochondria. (A) Total mitochondrial (mc) or mitoplasts' (mp) (prepared in the presence or absence of cantharidin) protein (10 μg) was separated by SDS/PAGE, and proteins were blotted onto nitrocellulose. Identical blots were probed with antibodies specific for the A, B, or C barley 14-3-3 isoforms, with anti-rabbit IgG-HRP conjugates as secondary antibody. Blots were developed by using the ECL system. (B) Protease protection assay carried out on mitochondria and mitoplasts to identify the subcompartmental location of the 14-3-3 proteins.