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. 2001 Mar 13;98(7):4249–4254. doi: 10.1073/pnas.061437498

Figure 2.

Figure 2

14-3-3 protein inhibits the mitoplastal ATP synthase. The respiration of isolated mitoplasts was measured in the presence of NADH as an electron donor. Trace A, the application of ADP converts the respiration of the mitoplasts from state 2 to state 3. Application of the uncoupler S13 leads to maximum oxygen uptake. Trace B, the application of 14-3-3 in combination with ADP inhibits the state 3 respiration. Trace C, the inhibition of the state 3 respiration by 14-3-3 can be overcome by the addition of excess phosphorylated NR-peptide, which competitively inhibits the binding of 14-3-3 to target proteins.