Figure 3.

The effect of 14-3-3 and phosphorylated NR-peptide on the rate of ATP hydrolysis of isolated broken chloroplasts. A batch of broken chloroplasts was split into three and treated with 14-3-3, phosphorylated NR-peptide, or buffer only. Aliquots were removed at the time intervals shown, and the rate of ATP hydrolysis was measured. ●, buffer control; ▴, 14-3-3; ♦, phosphorylated NR-peptide; ○, buffer control + phosphorylated NR-peptide; ▵, 14-3-3 + phosphorylated NR-peptide. The arrows illustrate the effect of adding phosphorylated NR-peptide to the buffer only and 14-3-3 batches. The experiment was repeated several times and a single representative experiment is shown.