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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: For Immunopathol Dis Therap. 2011;2(1):47–58. doi: 10.1615/forumimmundisther.v2.i1.60

FIGURE 2.

FIGURE 2

Locostatin disrupts binding of RKIP with Raf-1 without preincubation of RKIP with locostatin. RKIP (100 nM), immunoprecipitated FLAG-tagged Raf-1, and locostatin (200 µM; Loco) or DMSO alone (0.2%) as control (Ctl) were combined at the same time, then incubated for either (A) 6 h at 37°C or (B) 30 min at 4°C. After centrifugation and washing five times, the pellets were subjected to SDS-PAGE and Western blot analysis with an anti-RKIP antibody. Each Western blot is representative of at least three separate experiments.