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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: For Immunopathol Dis Therap. 2011;2(1):47–58. doi: 10.1615/forumimmundisther.v2.i1.60

FIGURE 5.

FIGURE 5

Locostatin modifies His86, a highly conserved residue in RKIP’s ligand-binding pocket. RKIP (20 µM) was incubated with locostatin (100 µM) or DMSO alone (0.1%) as control at 37°C for 24 h and then subjected to LC-MS/MS. Mass spectra are shown for the fragment 81-YREWHHFLVVNMK-93 from tryptic digestion of (A) the control RKIP sample and (B) the locostatin-treated RKIP sample. Tandem mass spectra are shown for the same tryptic fragment from (C) the control RKIP sample and (D) the locostatin-treated RKIP sample, with modified ions underlined.