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. 2011 Jun 8;31(23):8533–8542. doi: 10.1523/JNEUROSCI.1300-11.2011

Figure 2.

Figure 2.

Downregulation of ERK signaling in IQGAP1−/− mice. A, Hippocampal neurons derived from IQGAP1+/+ or IQGAP1−/− mice were treated with bicuculline (20 μm) and glycine (100 μm) for 3 min to activate Syn NMDARs. IQGAP1 and pERK colocalize in stimulated IQGAP1+/+ neurons. Double-label immunostaining for IQGAP1 (red, top), pERK1/2 (green, middle), and merge (yellow, bottom) revealed significantly reduced level of pERK1/2 in IQGAP1−/− neurons. Scale bar, 20 μm. B, Representative immunoblots showing decreased pERK1/2 (normalized to total ERK1/2) levels after stimulation (Stim) of NMDARs in IQGAP1−/− neurons with bicuculline and glycine. Quantification of fold change of immunoreactivity (pERK/ERK) from unstimulated control (Cont) is presented below (n = 3; *p < 0.05 compared to corresponding IQGAP1+/+). C, Representative immunoblots showing decreased c-fos versus actin levels in IQGAP1−/− versus IQGAP1+/+ neurons. Quantification of the fold change of immunoreactivity (c-fos/actin) from control is presented below (n = 3; *p < 0.05 compared to IQGAP1+/+). D, Representative immunoblots showing decreased pERK1/2 in the adult hippocampus after fear conditioning (FC) of IQGAP1−/− versus IQGAP1+/+ mice. Quantification of the fold change of immunoreactivity (pERK2/ERK2) is presented below (n = 4; ***p < 0.001 compared to IQGAP1+/+). E, The levels of c-fos were also reduced as revealed by decreased c-fos versus actin (representative immunoblots; top) and quantification of the fold change of immunoreactivity (c-fos/actin; bottom; n = 3–4; *p < 0.05 compared to IQGAP1+/+). Data are represented as mean ± SEM.