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. Author manuscript; available in PMC: 2011 Jun 23.
Published in final edited form as: Cell Cycle. 2008 Apr 16;7(13):2013–2020. doi: 10.4161/cc.7.13.6130

Figure 5.

Figure 5

Kif4A depletion led to reduced homologous recombination in cells. (A) Time scheme for the transfection of Kif4A shRNA and I-SceI expression plasmids. (B) Percentage of GFP positive population detected by FACS analysis with samples collected at 12, 24 or 48 hours after 2nd Kif4A shRNA/I-SceI expression. The results were showed separately in a histogram (C). (D) cell cycle profiles of cells harvested at 24, 48 or 72 hours after Kif4A shRNA transfection (with I-SceI co-transfected at 48 hrs) as in (A).