Table 2.
Inhibition of esterases in HNEC and HNBE cells (derived from data of Mutch et al 2007; Sato et al 2007a)
| Inhibitor | Target esterase |
Mean ± SEM % control activity |
|
|---|---|---|---|
| HNEC | NHBE | ||
| Paraoxon | CarbE, BChE, AChE | 25.0 ± 0.3 | 16.7 ± 0.7 |
| BNPP | CarbE | 24.9 ± 0.3 | 20.0 ± 3.8 |
| Iso-OMPA | CarbE | 29.3 ± 0.6 | 22.3 ± 3.8 |
| Eserine | AChE | 48.2 ± 0.3 | 28.7 ± 3.7 |
| PMB | ArE | 103.8 ± 0.8 | 90.3 ± 17.3 |
| EDTA | A-E | 105.8 ± 0.3 | 78.7 ± 4.7 |
Notes: data are expressed as percentage inhibition of control (no inhibitior) after incubation of cells with ciclesonide (5 × 10−6 mol/L) and esterase inhibitors (1 × 10−6 mol/L) (mean of duplicate estimations).
Abbreviations: HNEC, human bronchial epithelial cells; NHBE, normal human bronchial epithelial; CarbE, carboxylesterase; BChE, cholinesterase; AChE, acetylcholinesterase; ArE, arylesterase; A-E, A-esterase; BNPP, bis (p-nitrophenyl) phosphate; iso-OMPA, tetraisopropyl pyrophosphoramide; PMB, p-hydroxymercuribenzoate; EDTA, ethylenediaminetetraacetic acid.