FIGURE 1.

Functional characterization and localization of c-ETS1 element in cyclin E and CDK2 promoters. A and B, Huh7, HepG2, HeLa, and HEK293 cells were transfected with either pE-WT (A) or pCDK2-WT (B) reporter construct along with the expression vectors of SP1, and c-ETS1 and the relative CAT and luciferase activities were measured. C, pE-WT and pE-mut reporters were transfected in Huh7 cells along with SP1 and c-ETS1 expression vectors, and the relative CAT activity was measured. D, pCDK2-WT, pCDK2-prox.mut, pCDK2-dis.mut, and pCDK2-mut luciferase reporters were transfected in Huh7 cells along with the expression constructs of SP1 and c-ETS1, and the relative reporter activity was measured. Data shown in A–D are the mean ± S.D. of three independent experiments. The asterisk and number sign indicate statistically significant difference at p < 0.05 and p < 0.01, respectively.