FIGURE 2.

Effect of c-ETS1 on cyclin E and CDK2 expression and cell cycle progression. A and B, Huh7 and HeLa cells were transfected with SP1 and c-ETS1 expression vectors and the mRNA levels of cyclin E (A) and CDK2 (B) were measured by qRT-PCR. The sequences of primers are given in supplemental Table 1. C, Huh7 cells were transfected with either control or c-ETS1 expression vector and 48 h post-transfection, the cell extracts were processed for Western blotting with cyclin E, CDK2, c-ETS1, and GAPDH antibodies. D, 24 h post-transfected Huh7 cells were subjected to serum starvation for another 24 h and analyzed by flow cytometry. E and F, Huh7 cells transfected with indicated siRNA were analyzed for cyclin E and CDK2 transcripts level by qRT-PCR (E) or Western blotted (WB) for protein levels of cyclin E, CDK2, c-ETS1, and GAPDH (F). Data shown in A, B, and E are the mean ± S.D. of three independent experiments. The asterisk and number sign indicate statistically significant difference at p < 0.05 and p < 0.01, respectively.