FIGURE 6.

Induction of cyclin E and CDK2 transcription by HBx. A and B, Huh7, HepG2, HeLa, and HEK293 cells were transfected with either pE-WT (A) or pCDK2-WT (B) reporter constructs along with the expression vectors of c-ETS1 and HBx and the relative reporter activity was measured. C, Huh7 cells were transfected with either pCDK2-mut (bar) or pE-mut (line) along with the expression construct of HBx, and the relative reporter activity was measured. D–F, the relative mRNA levels of cyclin E (D) and CDK2 (E) genes were measured by qRT-PCR in Huh7 cells transfected with HBx along with either indicated siRNAs or c-ETS1 dominant negative. Data shown in A–F are the mean ± S.D. of three independent experiments. The asterisk and number sign indicate statistically significant difference at p < 0.05 and p < 0.01, respectively.