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. 2011 Apr 22;286(25):21982–21992. doi: 10.1074/jbc.M111.226795

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — A, Western blot analysis for glucokinase protein in clonal human beta cell lines 1.1B4, 1.1E7, 1.4E7, and PANC-1 cells. Liver from Wister rat was used as a positive control. B–E, immunohistochemistry (IHC) showing fluorescence staining for glucokinase protein in clonal 1.1B4, 1.1E7, 1.4E7, and PANC-1 parental cell lines. Slides were analyzed by fluorescence microscopy using an IX51 Olympus microscope (×20 magnification). F, the glucose-phosphorylating activities of parental and clonal cell lines. Soluble cytoplasmic fractions were assayed spectrophotometrically to determine the relative contribution of glucokinase and hexokinase to the total glucose-phosphorylating activities of the cells. Values are mean S.E. (n = 3). **, p < 0.01, ***, p < 0.001 when compared with PANC-1 cells.