FIGURE 8.

A, RT-PCR expression of genes of the K-ATP calcium (Kir6.2, Sur1) and L-type voltage-dependent calcium channel (VDCC) in 1.1B4, 1.1E7, 1.4E7, and PANC-1 (lanes 1–4). B–D, effects of acute (3-min) exposure to insulin secretagogues on intracellular Ca²⁺ in 1.1B4, 1.147, and 1.1E7 cell lines at 1.1 and 16.7 mm glucose (B), 1.1 mm glucose and 30 mm KCl (C), and 16.7 mm glucose and 20 mm alanine (D). Cells were exposed to secretagogues at time 0, and readings were taken every 2.5 s. Each data point represents mean ± S.E. (n = 6). RLU, relative light units.