FIGURE 3.

Time course of the GAL and GUS assembly reaction. GAL (A) and GUS (C) were synthesized in vitro for 30 min, and the reaction was terminated by adding tetracycline. Synthesis reactions were performed with 0.1 and 1 nm DNA with GAL and GUS, respectively. Terminated reaction mixtures were diluted 1-, 1.33-, 2-, and 4-fold for GAL, and 1-, 1.25-, 1.67-, 2-, and 2.5-fold for GUS with IVTT mixture and mixed with the fluorescent substrate. The time courses of hydrolysis reactions are shown. Fluorescence signals increase faster with higher concentrations. B, relationship between the initial velocity obtained from A and relative total monomer concentration.