FIGURE 6.

Time courses of the GUS assembly reaction analyzed by size exclusion chromatography. Time courses of monomer (A), dimer (B), and tretramer (C) formation. Data from the top show the time courses of 1 (gray circles), 1.5 (dark gray circles), and 2-fold (●) diluted terminated mix, respectively. The lines in A are those fitted with equation 11, and the lines in B and C are the joint lines. k₁ was estimated from the fitting, which was then converted into the appropriate dimension using the relationship between peak height and concentration (supplemental Fig. S4C).