FIGURE 1.

Dissociation of the CT holotoxin by reduced PDI. A, following ligands were perfused over a CT-coated SPR sensor slide at 37 °C: PDI, oxidized PDI (PDI + 1 mm GSSG), an anti-CTA antibody, an anti-CTB antibody, and an anti-KDEL antibody. B, after taking a baseline measurement corresponding to the mass of the sensor-bound holotoxin, reduced PDI (PDI + 1 mm GSH) was perfused over the CT-coated sensor slide at 37 °C. PDI was removed 400 s into the experiment and replaced with sequential additions of anti-PDI, anti-CTA, and anti-KDEL antibodies as indicated by the arrowheads.