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. 2011 Apr 29;286(25):22211–22218. doi: 10.1074/jbc.M110.180224

FIGURE 2.

FIGURE 2.

Reactivation of latent HIV-1 in J89GFP cells by oxamflatin, apicidin, scriptaid, and SAHA. A, J89GFP cells were treated with varying concentrations of HDACI (0–500 nm), and the percentage of cells expressing EGFP was quantitated after 24 h by FACs. Error bars represent S.E. from at least three independent experiments. B, J89GFP cells were treated with 200 nm HDACI, and the percentage of cells expressing EGFP was quantitated at different time intervals (0–75 h) by FACs. C, increase in HIV-1 RNA transcripts in cells treated with 200 nm HDACI for 24 h as measured by quantitative RT-PCR. Error bars represent S.E. from at least three independent experiments. The fold increase in transcript relative to untreated cells is indicated above each bar for all four HDACI. D, J89GFP cells were treated with varying concentrations of HDACI (0–2 μm), and the percentage of cells expressing EGFP was quantitated after 24 h by FACs. Error bars represent S.E. from at least three independent experiments.