FIGURE 5.

Activation of the PI3K/Akt signaling pathway by apicidin and oxamflatin. A, Western blot analysis of Akt phosphorylation in J89GFP cells treated with TNF-α (positive control) or with 500 nm apicidin, oxamflatin, SAHA, or scriptaid. Phosphorylated Akt and β-actin were detected using monoclonal antibodies specific for these proteins. B, J89GFP cells were treated with 500 nm HDACI with or without wortmannin (100 nm) or the Akt inhibitor IV (AI 4, 10 μm). The number of J89GFP cells expressing EGFP was quantitated after 24 h by FACs. Error bars represent S.E. from three independent experiments.