FIGURE 3.

p53 binds to the RGS13 promoter. A and B, nuclear extracts were isolated from LAD2 MCs (20 μg of protein) and aliquoted into reaction tubes containing biotinylated double-stranded oligonucleotides in the presence or absence of unlabeled competitor oligonucleotides as indicated. DNA-protein complexes were collected using streptavidin-coated beads, followed by assessment of protein binding by immunoblotting. WB, Western blot; mut, mutant. C, p53 binds to two p53 REs in the RGS13 promoter in vivo. Sheared chromatin from LAD2 cells was immunoprecipitated (IP) with the indicated antibodies, followed by amplification of RGS13 promoter sequences. Images represent two to three similar experiments.