FIGURE 4.

Doxorubicin-induced genotoxic stress reduces RGS13 expression in MCs. A, LAD2 cells were treated with doxorubicin (0.5 μm) for 12 h, followed by extraction of RNA and assessment of RGS13 quantities by real-time PCR. Expression is shown relative to untreated cells, set as 1 (mean ± S.E. of three independent experiments). **, p = 0.004 (paired t test). B and C, LAD2 cells were stimulated with the indicated concentrations of doxorubicin for various time periods. RGS13 quantities in cell lysates were determined by immunoblotting. Images represent two to three similar experiments. WB, Western blot.