FIGURE 1.

Cisplatin treatment causes Bim phosphorylation, leading to Bim-EL degradation in several ovarian cancer cell lines. A, Western blot analyses of Bim-EL expression in several ovarian cancer cell lines treated with 10 μm cisplatin for the indicated time periods. Actin was used as a loading control. In RMG-1 cells, two different exposures times were shown. B, MTT analyses of cell growth inhibition after 48 h of cisplatin treatment at the indicated concentrations. C, Western blot analyses of Bim-EL phosphorylation. RMG-1 cells were left untreated or treated with 10 μm cisplatin for 72 h. The whole cell lysates were collected and incubated with or without λ phosphatase in the presence (+) or absence (−) of the phosphatase inhibitor vanadate. The slower-migrating forms of Bim-EL are the result of phosphorylation. Actin was used as a loading control.