FIGURE 3.

Activation of ERK by cisplatin phosphorylates Bim-EL, leading to Bim-EL degradation and cisplatin resistance in ovarian cancer cells. A, Western blot analyses of Bim-EL, ERK, c-Jun, and CREB phosphorylation. RMG-1 cells were left untreated or treated with 10 μm cisplatin in the presence (+) or absence (−) of 10 μm U0126, 10 μm SB203580, or 10 μm SP600125 for 48 h. Actin was used as a loading control. B, Western blot analyses of Bim-EL expression. OVCA432 and OVCA420 cells were left untreated or treated with 10 μm cisplatin in the presence or absence of 10 μm U0126, 10 μm SB203580, or 10 μm SP600125 for 48 h. Actin was used as a loading control.