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. 2011 May 11;286(25):22384–22392. doi: 10.1074/jbc.M111.239566

FIGURE 4.

FIGURE 4.

Knockdown of Bim decreases cisplatin sensitivity, whereas knockdown of ERK decreases Bim-EL phosphorylation and degradation and increases cisplatin sensitivity. A, Western blot analyses of ERK, Bim-EL, and PARP expression. RMG-1 cells were transfected with control non-target siRNA or ERK siRNA. After 48 h, transfected cells were left untreated (−) or treated (+) with 10 μm cisplatin for 48 h. Actin was used as a loading control. B, Western blot analyses of Bim-EL and PARP levels. RMG-1 cells were transfected with control non-target siRNA or Bim siRNA. After 48 h, cells were left untreated (−) or treated (+) with 10 μm cisplatin for 48 h. Actin was used as a loading control. C, MTT analyses of growth inhibition. RMG-1 cells were transfected with control non-target siRNA, ERK siRNA, or Bim siRNA. 48 h later, cells were treated with cisplatin for 48 h.