FIGURE 6.

The Effects of ERK and Bim knockdown on proteasome inhibitor, cisplatin, and their combination-induced growth inhibition. A, Western blot analyses of Bim-EL and ERK expression. RMG-1 cells were transfected with control non-target siRNA, ERK siRNA, or Bim siRNA. After 48 h, cells were left untreated or treated with 10 μm cisplatin in the presence (+) or absence (−) of 250 nm MG132 for 48 h. Actin was used as a loading control. B, MTT analyses of growth inhibition. RMG-1 cells were transfected with control non-target siRNA, ERK siRNA, or Bim siRNA. 48 h later, cells were left untreated or treated with 10 μm cisplatin in the presence or absence of 250 nm MG132 for 48 h.