TABLE 1.

Analysis of the oligomeric state and molecular shape of mBMAL1 and mCRYCCtail fragments by analytical ultracentrifugation

Proteina	sb	RHc	RH globular	RH GuHCl-unfolded	MWd	Oligomeric state
	S	nm	nm	nm	Da
mCRY1CCT	1.146	3.26	1.91	3.43	14433	Monomeric
mCRY2CCT	1.176	2.50	1.79	3.12	12087	Monomeric
BMAL490	1.322	2.62	1.90	3.41	14264	Monomeric
BMAL577	0.667	1.98	1.35	2.03	5482	Monomeric

^a The concentration of mCRY1/2 and BMAL490 was adjusted to A₂₈₀ = 0.5 (corresponds to 0.07 mm mCRY1, 0.17 mm mCRY2, 0.06 mm BMAL490). The BMAL577 concentration was 0.2 mm. BMAL490 was also shown to be monomeric at A₂₈₀ = 5 corresponding to 0.6 mm BMAL490. mCRY1/2CCT = mCRY1/2 coiled-coil-tail fragment. BMAL490/577 = mBMAL1-(490/577–625) fragment.

^b s[S] = sedimentation coefficient in Svedberg units. The s values are normalized to 20 °C and water.

^c R_H = hydrodynamic (Stokes) radius.

^d Sequence molecular weight, calculated as described under “Experimental Procedures.”