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Protein interaction between p62/SQSTM1 and proteasomes detected by the PLA. SK-N-SH cells control (A, Ct) or treated for 24 h with 100 μm chloroquine (B, CQ) or 25 nm epoxomicin (C, Epx) were co-incubated with a proteasome antibody (Rpt6/S8) and a p62/SQSTM1 antibody. Nuclear staining, DAPI, and PLA signal, Texas Red. Scale bars, 7 μm.
